TEM of Cultured Cells on Coverslips

Estimation of the Cultured Cells’ Volume and Surface Area: Application of Stereological Methods on Vero Cells Infected by Rubella Virus

Background: Morphological changes of the cells infected with rubella virus cannot be observed easily. Estimation of the size of the cultured cells can be a valuable parameter in this condition. This study was conducted to find answers to the following questions:How much time after infection with rubella virus, the volume and surface area of the Vero cells and their nuclei get started to change?...

The Effect of Culture System on Benzaldehyde Biotransformation by Cultured Cells of Silybum marianum (L.) Gaertn.

      The abilities of Silybum marianum cell culture to biotransform benzaldehyde to benzyl alcohol in three culture systems were compared. Callus cultures of Silybum marianum were established from seedlings, and healthy suspensions and immobilized cultures grown on the Murashige and Skoog medium. S. marianum cells were immobilized in both agar beads and fiber cotton ...

Increased mir33 Expression in Expanded Hematopoietic Stem Cells Cultured on Adipose Stem Cells Feeder layer

Bachgroun: Hematopoietic stem cell derived from umbilical cord blood (UCB) has been used for regenerative medicine in hematological abnormalities. MicroRNAs are important regulators of gene expression that control both physiological and pathological processes such as development of tissue and cancer. Some studies have shown that miR-33, has a critical role in control of self-renewal cells. He...

Mounting technique allows observation of immuno-labeled cells on plastic coverslips.

CFU-GM Like Colonies Derived from Embryonic Stem Cells Cultured on the Bone Marrow Stromal Cells

The aim of this study was to isolate mouse embryonic stem cells from late blastocyst stage embryos and to use them as a model system for the study of hematopoietic induction outside the embryo by coculturing of embryonic stem cells with bone marrow stromal cells. Blastocyst stage embryos from pregnant NMRI mice were obtained and cultured for 1-2 days in DMEM medium. The inner cell masses formed...